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Literature summary extracted from

  • Sunita, S.; Purta, E.; Durawa, M.; Tkaczuk, K.L.; Swaathi, J.; Bujnicki, J.M.; Sivaraman, J.
    Functional specialization of domains tandemly duplicated within 16S rRNA methyltransferase RsmC (2007), Nucleic Acids Res., 35, 4264-4274.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.172 the rsmC gene, cloned into pCA24N vector with a noncleavable N-terminal His6 tag Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.172 hanging drop vapor diffusion method, crystal structure of RsmC refined to 2.1 A resolution, reveals two homologous domains tandemly duplicated within a single polypeptide. Characterization of the function of the individual domains and identification of key residues involved in binding of rRNA and S-adenosyl-L-methionine, and in catalysis. It is discovered that one of the domains is important for the folding of the other. RsmC can be regarded as a model system for functional streamlining of domains accompanied by the development of dependencies concerning folding and stability Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.172 S-adenosyl-L-methionine + guanine1207 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.172 Escherichia coli P39406
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.172 purification and refolding of C-RsmC from inclusion bodies Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.172 S-adenosyl-L-methionine + guanine1207 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.172 RsmC
-
 Escherichia coli